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Supervisor: Prof. Jean-François Collet
Supervisor: Dr. Jean-Pierre Simorre
Supervisor: Dr. Margherita Sosio
I graduated with a BSc-MSc Dual Degree (2023) from the Indian Institute of Science Education and Research (IISER), Mohali, Punjab, India. I obtained a major degree in Biological Sciences, and a minor degree in Chemical Sciences. I did my master’s thesis in a microbiology lab, to study the role of a small RNA in the regulation of Sugar acid metabolism in Escherichia coli. I am interested in studying about ‘the microbes’, their regulatory systems and their interactions with other organisms.
I did a summer internship in a ‘bacterial genetics and physiology’ lab at IISER Mohali, where I had hands-on-experience of various techniques of molecular biology. In 2022, I did another summer internship at Indian Institute of Science (IISc), Bangalore, India, where I worked in a ‘Caenorhabditis elegans lab’. My project aimed to understand the volatile profile of C. elegans microbiome members. I also did another project at IISc focused on understanding the swarming motility in Pseudomonas aeruginosa mutants in different growth conditions.
I am delighted to join the lab of Prof. Jean-François Collet as a PhD student at Université catholique de Louvain, Belgium as an MSCA fellow in the BREAKthrough consortium. I am looking forward to understanding the molecular mechanisms which maintain the integrity of bacterial cell envelope and how bacteria respond to different stress conditions. I am interested in identifying the inhibitors of protein machineries that help maintain the outer membrane in order to make Gram-negative bacteria sensitive to new antibiotics.
Antimicrobial resistance, which is caused by multi-drug-resistant bacterial pathogens is a global health emergency. Gram-negative bacteria notably hinder effective treatment because of their impermeable outer membrane (OM). This project aims to investigate the interaction between lipoproteins and the beta-barrel Assembly Machinery (BAM) using various in vivo and in vitro approaches. These approaches include site-specific photocrosslinking, pulldowns, protein purification and microfluidics. One objective of the BREAKthrough project is to destabilise the OM to make Gram-negative bacteria sensitive to new antibiotics. In this context, the potential of poorly characterized peptidoglycan transpeptidases as drug targets will be explored.
Funded by the European Union. Views and opinions expressed are however those of the author(s) only and do not necessarily reflect those of the European Union or the European Research Executive Agency. Neither the European Union nor the granting authority can be held responsible for them.
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